| Material | serum 1 ml (promptly centrifuged, pipetted off and at least refrigerated – preferably frozen) |
| Method | CLIA
ELISA (only cat) |
| Duration | 1 day |
| Remark | - In case an insulinoma is suspected; concentration is only diagnostically conclusive if glucose is determined at the same time.
- 12-hour fasting period prior to sampling no longer recommended for horses, but no concentrated feed or cereal grains – only hay.
- The serum must immediately be centrifuged, pipetted and cooled before shipping.
- Dog: Determine insulin/glucose ratio or AIGR (amended insulin/glucose ratio). An insulin/glucose ratio < 52 or an AIGR < 30 are considered normal.
- Equine metabolic syndrome (EMS): EMS leads to a disorder of carbohydrate and fat metabolism with insulin dysregulation (ID). Increased insulin secretion (partly) compensates for decreased insulin efficiency. Insulin-dysregulated horses therefore have considerably increased fasting insulin levels. At the same time, fasting glucose is physiological (compensated) or elevated (not compensated). Further tests: glucose tolerande test, combined glucose insulin test and oral glucose test (see Function Tests).
- Simultaneous measurement of the glucose level makes it possible to calculate the proxies
insulin/glucose ratio,
reciprocal inverse square of insulin (RISQI) - “insulin sensitivity” and
modified insulin-to-glucose ratio (MIRG) -“ß-cell function (pancreas)”.
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