TRH Stimulation Test with ACTH (horse) (2x ACTH)

LABOKLIN Service ID: 1186


Diagnosis

  • PPID (Cushing‘s disease)
  • Test with high sensitivity and specificity; indications: if the results of ACTH determination or of the suppression test do not correlate with clinical findings or are not conclusive.

Material

EDTA plasma 2 x 0.5 ml (centrifuged, pipette off and cool soon after sampling)

Execution

  • First blood collection = baseline value
  • Slow injection of 1 mg of TRH i.v.
  • Second sample 10 min post TRH injection = stimulation value

Parameter

ACTH

Method

CLIA

Species list

Horse

Duration

1 day

Assessment

Cut off value 10 min after stimulation: < 110 pg/ml;
borderline: 110 – 200 pg/ml; positive: > 200 pg/ml
These values are valid for mid-November to mid-July; so far, this test is not recommended for mid-July to mid-November.