LABOKLIN Service ID: 1186
| Diagnosis | - PPID (Cushing‘s disease)
- Test with high sensitivity and specificity; indications: if the results
of ACTH determination or of the suppression test do not correlate
with clinical findings or are not conclusive.
|
| Material | EDTA plasma 2 x 0.5 ml (centrifuged, pipette off and cool soon after sampling) |
| Execution | - first blood collection = baseline value
- Directly followed by slow i.v. injection of 1 mg TRH for horses >250 kg (horses <250 kg: 0.5 mg)
- Second blood collection after exactly 10 min = stimulation value
Third blood sample can additionally be taken 30 minutes after TRH administration
|
| Parameter | ACTH |
| Method | CLIA |
| Species list | Horse |
| Duration | 1 day |
| Assessment | cut off value 10 min after stimulation: < 100 pg/ml;
borderline: 100-200 pg/ml; positive: > 200 pg/ml
Cut off 30 min after stimulation: < 40 pg/ml;
borderline: 40-90 pg/ml; positive: > 90 pg/ml
These values apply to the months of January to June.
The stimulation test is not recommended from July to December. If the result is negative during this period (< 100 pg/ml after 10 min, < 40 pg/ml after 30 min), the diagnosis of PPID is very unlikely. |
