| Material | serum or heparin plasma 0.5 ml |
| Method | line immunoassay |
| Duration | 3 working days |
| Remark | - The Borrelia blot is used to clarify questionable antibody concentrations and to distinguish between vaccine-induced and infection-induced antibodies.
- The blot also detects antibodies against the VlsE protein and C6 peptide. VlsE (variable major protein-like sequence, expressed) and its subunit C6 are highly immunogenic surface antigens that express Borrelia during active reproduction. There is no recombination of the VlsE molecule in vitro or in the Borrelia residing in the tick, the detection of antibodies against the VlsE molecule therefore indicates an infection that has previously occurred.
- The blot can be carried out at the earliest from the 3rd week after infection onwards.
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