TRH Stimulation Test with ACTH (horse) (2x ACTH)

LABOKLIN Service ID: 1186


Diagnosis

  • PPID (Cushing‘s disease)
  • Test with high sensitivity and specificity; indications: if the results of ACTH determination or of the suppression test do not correlate with clinical findings or are not conclusive.

Material

EDTA plasma 2 x 0.5 ml (centrifuged, pipette off and cool soon after sampling)

Execution

  • first blood collection = baseline value
  • horses > 250 kg: slow injection of 1 mg of TRH i.v. (horses < 250 kg: 0.5 mg)
  • second sample exactly 10 min post TRH injection = stimulation value

Parameter

ACTH

Method

CLIA

Species list

Horse

Duration

1 day

Assessment

cut off value 10 min after stimulation: < 100 pg/ml;
borderline: 100 – 200 pg/ml; positive: > 200 pg/ml
These values are valid for the months of January to June. From July to December, the test can only be used to identify healthy horses, as many false positive results may occur during these months.