LABOKLIN Service ID: 1107
Indications:
- Treatment failure in a dog with confirmed leishmaniosis that is being treated with allopurinol
- immediately upon initial diagnosis to determine further treatment
| Material | bone marrow (preferred), tissue (lymph node aspirate, spleen, skin scabs), EDTA blood 1 ml |
| Method | Culture
+ realtime PCR (quantitative detection of Leishmania infantum)
+ droplet digital PCR (quantification of the METK gene) |
| Species list | dog |
| Duration | 5-6 working days |
| Remark | - Sample material: Bone marrow is preferred, but skin scabs (e.g. from lesions on the edge of the ear) are also suitable as they often have high concentrations of the pathogen. The pathogen concentration in EDTA blood is usually lower.
- Culture enrichment is carried out in advance. However, if the pathogen concentration is low, it is still possible that the required amount of pathogens cannot be achieved despite growing a culture (especially in EDTA blood samples).
- Detection of allopurinol resistance is done by quantifying the METK gene. However, droplet digital PCR (ddPCR) to determine the copy number of the METK gene is only possible if Leishmania infantum has been detected in sufficient quantities in realtime PCR. If ddPCR cannot be performed, a lower price will be charged.
- An METK gene copy number < 3.0 is currently considered a strong indicator of resistance, while a copy number < 4.0 is considered suspicious.
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