TRH Stimulation Test with ACTH (horse) (2x ACTH)

LABOKLIN Service ID: 1186


Diagnosis

  • PPID (Cushing‘s disease)
  • Test with high sensitivity and specificity; indications: if the results of ACTH determination or of the suppression test do not correlate with clinical findings or are not conclusive.

Material

EDTA plasma 2 x 0.5 ml (centrifuged, pipette off and cool soon after sampling)

Execution

  • first blood collection = baseline value
  • Directly followed by slow i.v. injection of 1 mg TRH for horses >250 kg (horses <250 kg: 0.5 mg)
  • Second blood collection after exactly 10 min = stimulation value
    • Third blood sample can additionally be taken 30 minutes after TRH administration

Parameter

ACTH

Method

CLIA

Species list

Horse

Duration

1 day

Assessment

cut off value 10 min after stimulation: < 100 pg/ml;
borderline: 100-200 pg/ml; positive: > 200 pg/ml
Cut off 30 min after stimulation: < 40 pg/ml;
borderline: 40-90 pg/ml; positive: > 90 pg/ml
These values apply to the months of January to June. The stimulation test is not recommended from July to December. If the result is negative during this period (< 100 pg/ml after 10 min, < 40 pg/ml after 30 min), the diagnosis of PPID is very unlikely.