Establishment of an agamid cell line and isolation of adenoviruses from central bearded dragons (Pogona vitticeps)

J. Vet. Diagn. Invest. 2014;  DOI: 10.1177/1040638714523615.

(Ball I, Hoferer M, Marschang RE)

Cloacal Aerobic Bacterial Flora and Absence of Viruses in Free-Living Slow Worms (Anguisfragilis), Grass Snakes (Natrixnatrix) and European Adders (Viperaberus) from Germany

EcoHealth ,2014 International Association for Ecologyand Health


(Volker Schmidt, Ronja Mock, Eileen Burgkhardt, Anja Junghanns, Falk Ortlieb, Istvan Szabo, Rachel Marschang, Irmgard Blindow and Maria-Elisabeth Krautwald-Junghanns)


Disease problems caused by viral or bacterial pathogens arc common in reptiles kept in captivity. There is no information available on the incidence of viral pathogens or the physiological cloacal bacterial flora of common free-living reptiles in Germany. Therefore, 56 free-living reptiles including 23 European adders {Vipera bents), 12 grass snakes {Natrix natrix) and 21 slow worms (Anguis fragilis) were investigated on the island Uiddenscc in northeastern Germany. Pharyngeal and cloacal swabs were taken immediately after capture. Bacteriological examination was performed from the cloacal swabs to study the aerobic cloacal flora. Molecular biological examination included amplification of DNA or RNA from adeno-, rana- and fcrlaviruses as well as culturing on Russell's viper heart cells for virus isolation. Salmonella spp. were isolated from European adders but not from the other reptiles examined. The minimal inhibitory concentration was determined from the isolated Salmonella spp. However, some potentially human pathogenic bacteria, such as Proteus vulgaris, Aeromonas hydrophila, Klebsiella pneumoniae and Escherichia coli were isolated. Viruses were not detected in any of the examined reptiles. To the authors' best knowledge, the present study is the first survey of viral pathogens in free-living snakes and slow worms in Germany and the first survey of cloacal aerobic bacterial flora of slow worms.

Current Therapy in Reptile Medicine and Surgery.

Clinical Virology. In: Mader DR, Divers SJ (eds.).  Elsevier, Pages 368-381; 

ISBN: 978-1-4557-0893-2

(Marschang RE)