{"id":1426087,"date":"2020-07-06T12:41:41","date_gmt":"2020-07-06T10:41:41","guid":{"rendered":"https:\/\/staging-wp-int.laboklin.com\/specialist-information\/preanalytics\/histology-cytology\/"},"modified":"2026-03-18T12:15:02","modified_gmt":"2026-03-18T11:15:02","slug":"histologie-immunohistologie-cytologie","status":"publish","type":"page","link":"https:\/\/laboklin.com\/fr\/infotheque\/preanalytique\/histologie-immunohistologie-cytologie\/","title":{"rendered":"Histologie\/Immunohistologie\/<br>Cytologie"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row type=\u00a0\u00bbvc_default\u00a0\u00bb css=\u00a0\u00bb.vc_custom_1617779413041{margin-bottom: 30px !important;}\u00a0\u00bb][vc_column][vc_column_text css=\u00a0\u00bb\u00a0\u00bb]When sending tissue samples for histopathological and immunohistological examination, the following points must be observed:<\/p>\n<ul>\n<li>artefact-free sampling of a typical lesion of sufficient size (diameter &gt; 05 cm)<\/li>\n<li>immediate fixation (4% formaldehyde \u2259 10% formalin)<\/li>\n<li>preparation of an anamnesis including diagnostic task and clinical picture<\/li>\n<li>shipment in a suitable container (available from us free of charge)<\/li>\n<li>Immunohistochemistry can always be done after histopathology with the material supplied.<\/li>\n<\/ul>\n<h5>Detailed explanations:<\/h5>\n<p>As a sample, a representative piece of tissue free of preparation artefacts (e. g. disruption, squashing, electrocoagulation) should be taken. The diameter of the sample should not be less than 0.6 cm. An exception to this are samples which, for technical reasons, cannot be obtained otherwise (such as endoscopically taken stomach biopsies). Furthermore, it should be borne in mind that samples which are too small only provide little information, whereas samples that are too large cannot be fixed properly. Pieces of tissue with an edge length of 1 cm are recommended. However, this might vary depending on the lesion to be examined, the sampling site and diagnostic task.<\/p>\n<p>Small lesions should be placed centrally so they are not overlooked and thus truncated during preparation. If in doubt, several samples should be collected.[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbvc_default\u00a0\u00bb][vc_column][vc_tta_tour style=\u00a0\u00bbmodern\u00a0\u00bb shape=\u00a0\u00bbsquare\u00a0\u00bb controls_size=\u00a0\u00bbsm\u00a0\u00bb active_section=\u00a0\u00bb1&Prime;][vc_tta_section title=\u00a0\u00bbPr\u00e9l\u00e8vements cutan\u00e9s\u00a0\u00bb tab_id=\u00a0\u00bb1581887762168-425b7db1-c4e02de7-add4&Prime;][vc_column_text css=\u00a0\u00bb\u00a0\u00bb]<\/p>\n<h3>Pr\u00e9l\u00e8vements cutan\u00e9s<\/h3>\n<p>Les \u00e9chantillons cutan\u00e9s \u00e0 envoyer sont des biopsies ponctionn\u00e9es de toutes les couches cutan\u00e9es d&rsquo;un diam\u00e8tre \u2265 0,6 cm. Il convient de choisir des modifications primaires provenant de plusieurs localisations. La zone biopsi\u00e9e ne doit pas avoir faire l&rsquo;objet de traitements pr\u00e9alables tels que grattage ou rasage.[\/vc_column_text][\/vc_tta_section][vc_tta_section title=\u00a0\u00bbCytology\u00a0\u00bb tab_id=\u00a0\u00bb1581887762192-7e387ca9-50822de7-add4&Prime;][vc_column_text css=\u00a0\u00bb\u00a0\u00bb]<\/p>\n<h3>Cytology<\/h3>\n<p>Les pr\u00e9l\u00e8vements peuvent \u00eatre effectu\u00e9s principalement par ponction (avec ou sans aspiration) ou par frottis. L&rsquo;aspiration \u00e0 l&rsquo;aiguille fine est la technique la plus courante. On utilise une canule fine (G22 \u2013 G27) avec ou sans seringue (needle-alone). Une fois la seringue mise en place, une d\u00e9pression est cr\u00e9\u00e9e et le tissu est perfor\u00e9 plusieurs fois dans diff\u00e9rentes directions. Avant de retirer la canule, la d\u00e9pression doit \u00eatre supprim\u00e9e afin d&rsquo;\u00e9viter que le mat\u00e9riel ne reflue dans la seringue. Le mat\u00e9riel pr\u00e9lev\u00e9 est ensuite transf\u00e9r\u00e9 de la canule vers un porte-objet \u00e0 l&rsquo;aide d&rsquo;une surpression. Une deuxi\u00e8me lame est pos\u00e9e \u00e0 angle droit \u00e0 plat sur celle-ci, puis retir\u00e9e d\u00e9licatement sur le c\u00f4t\u00e9 (vid\u00e9os dans la rubrique Informations sp\u00e9cialis\u00e9es \/ Tutoriels vid\u00e9o). Si le mat\u00e9riel est plus liquide, la deuxi\u00e8me lame est retir\u00e9e \u00e0 un angle oblique (45\u00b0) \u2013 comme pour un frottis sanguin.<br \/>\nPour l&rsquo;examen cytologique des ponctions, excr\u00e9tions ou s\u00e9cr\u00e9tions, les liquides pr\u00e9lev\u00e9s sont centrifug\u00e9s \u00e0 2500 &#8211; 3000 tours\/minute pendant trois \u00e0 cinq minutes. Le surnageant est d\u00e9cant\u00e9 et le d\u00e9p\u00f4t est soigneusement \u00e9tal\u00e9 comme pour un frottis sanguin, puis s\u00e9ch\u00e9 \u00e0 l&rsquo;air libre avant d&rsquo;\u00eatre envoy\u00e9. Veuillez indiquer sur la demande d&rsquo;analyse s&rsquo;il s&rsquo;agit d&rsquo;un frottis de s\u00e9diment ou d&rsquo;un frottis natif. Si les pr\u00e9l\u00e8vements sont envoy\u00e9s directement, il convient d&rsquo;utiliser des tubes non rev\u00eatus et des tubes EDTA comme r\u00e9cipients pour les \u00e9chantillons.<br \/>\nPour la cytologie bronchique, conjonctivale et vaginale, l&rsquo;\u00e9couvillon pr\u00e9lev\u00e9 (Cytobrush) doit \u00eatre d\u00e9roul\u00e9 sur une lame porte-objet et non \u00e9tal\u00e9.<br \/>\nTous les frottis doivent g\u00e9n\u00e9ralement \u00eatre s\u00e9ch\u00e9s \u00e0 l&rsquo;air libre, mais ne doivent pas \u00eatre fix\u00e9s lorsqu&rsquo;ils sont envoy\u00e9s. Si vous le souhaitez, les frottis peuvent \u00eatre color\u00e9s directement dans votre cabinet (attention : sans lamelle couvre-objet).<br \/>\nLe plus important est de r\u00e9aliser un frottis fin \u00e0 partir d&rsquo;une seule couche cellulaire (monocouche). Les frottis trop \u00e9pais sont la cause la plus fr\u00e9quente d&rsquo;une qualit\u00e9 m\u00e9diocre, voire d&rsquo;une impossibilit\u00e9 d&rsquo;\u00e9valuation.[\/vc_column_text][\/vc_tta_section][\/vc_tta_tour][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row type=\u00a0\u00bbvc_default\u00a0\u00bb css=\u00a0\u00bb.vc_custom_1617779413041{margin-bottom: 30px !important;}\u00a0\u00bb][vc_column][vc_column_text css=\u00a0\u00bb\u00a0\u00bb]When sending tissue samples for histopathological and immunohistological examination, the following points must be observed: artefact-free sampling of a typical lesion of sufficient size (diameter &gt; 05 cm) immediate fixation (4% formaldehyde \u2259 10% formalin) preparation of an anamnesis including diagnostic task and clinical picture shipment in a suitable container&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1383542,"menu_order":6,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"LABOKLIN Preanalytics: Histology\/Immunhistology\/Cytology","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1426087","page","type-page","status-publish","hentry","description-off"],"_links":{"self":[{"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/pages\/1426087","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/comments?post=1426087"}],"version-history":[{"count":5,"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/pages\/1426087\/revisions"}],"predecessor-version":[{"id":1543054,"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/pages\/1426087\/revisions\/1543054"}],"up":[{"embeddable":true,"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/pages\/1383542"}],"wp:attachment":[{"href":"https:\/\/laboklin.com\/fr\/wp-json\/wp\/v2\/media?parent=1426087"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}