Histology/Immunohistology

When submitting tissue samples for pathohistological and immunohistochemical examinations, the following points must be observed:

  • Artefact-free taking of a typical lesion sufficient in size (diameter > 0.5 cm)
  • Immediate fixation (4% neutral buffered formaldehyde = 10% formalin)
  • Preparation of an anamnesis including objective and clinical picture
  • Shipment in a suitable container (available from us free of charge)
  • Immunohistochemistry can always be done after histopathology with the material supplied

Detailed explanations:

As a sample, a representative piece of tissue free of preparation artefacts (e. g. disruption, squashing, electrocoagulation) should be taken. The diameter of the sample should not be less than 0.5 cm. An exception to this are samples which, for technical reasons, cannot be obtained otherwise (such as endoscopically taken stomach biopsies). Furthermore, it should be borne in mind that samples which are too small only provide little information, whereas samples that are too large cannot be fixed properly. Pieces of tissue with an edge length of 1 cm are recommended. However, this might vary depending on the lesion to be examined, the sampling site and the objective.

Small lesions should be placed centrally so they are not overlooked and thus truncated during preparation. If in doubt, several samples should be collected.