Analysis on Multidrug-resistant bacteria

LABOKLIN Service ID: 1640

This profile is used to specifically screen for bacteria that are resistant to critically important antibiotics in order to identify
asymptomatic carriers (e.g. therapy dogs in hospitals and care facilities). Particularly critical resistances occur in methicillinresistant staphylococci (mainly Staphylococcus aureus and S. pseudintermedius in dogs), vancomycin-resistant enterococci mainly Enterococcus faecium and E. faecalis) and enterobacteria resistant to 3rd and 4th generation cephalosporins and to carbapenem antibiotics.

Material

4 x swab with medium

  • swab 1: nasal-buccal
  • swab 2: skin (armpit or groin) or conjunctiva
  • swab 3: pooled swab from the animal’s environment (dog basket + food bowl + floor)
  • swab 4: rectal swab

Parameter

MRSA, VRE, ESBL, Carbapenemase producing bacteria

Method

culture, microdilution

Duration

3 - 4 days

Remark

  • Phenotypic detection of resistance.
  • Swabs 1 – 3 are used to detect MRSA; swab 4 is used to detect vancomycin-resistant enterococci and multidrug-resistant gramnegative bacteria.
  • The sampling sites are recommendations. If other sites are prescribed by the hospital or the respective facility, please follow these recommendations.
  • The profile provides phenotypic detection of resistance of selected bacterial isolates. It does not test for resistance genes by molecular biology.
  • The test result reflects the current colonisation of the animal, so it needs to be considered whether regular screenings should be carried out.