{"id":1418201,"date":"2022-09-06T10:54:57","date_gmt":"2022-09-06T08:54:57","guid":{"rendered":"https:\/\/staging-wp-int.laboklin.com\/products\/haematology-clin-chemistry-endocrinology\/function-tests-calculation-formulas\/acth-stimulationtest\/"},"modified":"2025-11-20T10:30:40","modified_gmt":"2025-11-20T09:30:40","slug":"acth-stimulation-test","status":"publish","type":"page","link":"https:\/\/laboklin.com\/dk\/products\/haematology-clin-chemistry-endocrinology\/function-tests-calculation-formulas\/acth-stimulation-test\/","title":{"rendered":"ACTH Stimulation Test"},"content":{"rendered":"

ACTH Stimulation Test<\/h3>

LABOKLIN Service ID: 141<\/span><\/p>


<\/p>
Diagnosis<\/strong><\/td>

  • Diagnosis of hypoadrenocorticism (Addison\u2019s disease)<\/li>
  • Clarification of iatrogenic Cushing\u2019s syndrome<\/li>
  • Suspected Cushing\u2019s syndrome<\/li>
  • Horse: clarification of hypoadrenocorticism (Addison\u2019s disease)<\/li><\/ul><\/p><\/td><\/tr>
Material<\/strong><\/td>

Serum 2 x 0.5 ml<\/p><\/td><\/tr>

Execution<\/strong><\/td>

  • First blood collection = baseline value<\/li>
  • Dog:<\/strong> injection of 5 \u03bcg ACTH\/kg as Cosacthen\u00ae i.v.\/(i.m.)<\/li>
  • Cat: <\/strong>injection of 5 \u03bcg\/kg Cosacthen\u00ae i.v.\/(i.m.)<\/li>
  • Second blood collection (= stimulation value) in dog, cats one hour post injection<\/li>
  • Guinea pig:<\/strong> baseline value cortisol, 2 IU ACTH\/kg, i.v.\/i.m., second sample collection after 4 hours
  • Horse: <\/strong>injection of 100 IU ACTH i.v. <\/li>
  • Second blood collection 2 hours post ACTH administration = stimulation value in horses<\/li> <\/ul><\/p><\/td><\/tr>
Parameter<\/strong><\/td>

Cortisol<\/p><\/td><\/tr>

Method<\/strong><\/td>

Chemiluminescence assay (CLIA)<\/p><\/td><\/tr>

Species list<\/strong><\/td>

Dog, cat, horse, guinea pig, others on request<\/p><\/td><\/tr>

Duration<\/strong><\/td>

Reporting of results on the day the sample is received (Mon\u2013Sat)<\/p><\/td><\/tr>

Assessment<\/strong><\/td>

  • To assess cortisol concentration, previous glucocorticoid treatment should be ruled out (except when clarifying iatrogenic Cushing\u2019s syndrome).<\/li>
  • Clinical issue diagnosis of hypoadrenocorticism (Addison\u2019s\r\ndisease): Dogs<\/strong> with hypoadrenocorticism typically have concentrations in the lower reference range or below. Stimulation typically results in no or only a slight increase in cortisol concentrations. The intake of steroids can produce similar results.<\/li>
  • Clinical issue diagnosis of Cushing\u2019s syndrome: Cushing\u2019s syndrome may be considered if the clinical signs are consistent and stimulation values are elevated. In dogs, the ACTH stimulation test is considered more specific but less sensitive than the lowdose dexamethasone suppression test for diagnosing Cushing\u2019s syndrome. False negative results are common, especially if Cushing\u2019s syndrome is caused by an adrenal tumour.<\/li>
  • Other underlying conditions (e.g. diabetes mellitus) can also lead to elevated stimulation values.<\/li>
  • Clinical issue therapy monitoring for Cushing\u2019s syndrome: When treating with trilostane (Vetoryl\u00ae), the target stimulation value is between 20 ng\/ml and 50 ng\/ml. For evaluating this treatment, \u201cTrilostane Therapy Control\u201d (see Endocrinology) is also available.<\/li>
  • Horse:<\/strong> In healthy animals, the cortisol level increases by approximately 80%; horses with hypoadrenocorticism (Addison\u2019s disease) show very low baseline values which do not or only slightly increase after stimulation.<\/li>
  • Guinea pig:<\/strong> for therapy monitoring of Cushing\u2019s disease; at the time of diagnosis, it is not possible to differentiate between pituitary and adrenal Cushing\u2019s syndrome; there are no reference values available.<\/li><\/ul><\/p><\/td><\/tr>
Remark<\/strong><\/td>

In guinea pigs, an ACTH stimulation test can also be performed using saliva (servie ID 1329 Salivary Cortisol Stimulation Test). Sample tubes are provided; saliva samples are analysed using ELISA detection method.<\/p><\/td><\/tr><\/table>\n","protected":false},"excerpt":{"rendered":"

ACTH Stimulation TestLABOKLIN Service ID: 141DiagnosisDiagnosis of hypoadrenocorticism (Addison\u2019s disease)Clarification of iatrogenic Cushing\u2019s syndromeSuspected Cushing\u2019s syndromeHorse: clarification of hypoadrenocorticism (Addison\u2019s disease)MaterialSerum 2 x 0.5 mlExecution First blood collection = baseline value Dog: injection of 5 \u03bcg ACTH\/kg as Cosacthen\u00ae i.v.\/(i.m.) Cat: injection of 5 \u03bcg\/kg Cosacthen\u00ae i.v.\/(i.m.)Second blood collection (= stimulation value) in dog, cats…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1383132,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"footnotes":""},"class_list":["post-1418201","page","type-page","status-publish","hentry","description-off"],"_links":{"self":[{"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/pages\/1418201","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/comments?post=1418201"}],"version-history":[{"count":1,"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/pages\/1418201\/revisions"}],"predecessor-version":[{"id":1524240,"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/pages\/1418201\/revisions\/1524240"}],"up":[{"embeddable":true,"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/pages\/1383132"}],"wp:attachment":[{"href":"https:\/\/laboklin.com\/dk\/wp-json\/wp\/v2\/media?parent=1418201"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}