{"id":1418200,"date":"2022-09-06T10:54:57","date_gmt":"2022-09-06T08:54:57","guid":{"rendered":"https:\/\/staging-wp-int.laboklin.com\/products\/haematology-clin-chemistry-endocrinology\/function-tests-calculation-formulas\/acth-stimulationtest\/"},"modified":"2025-11-20T10:30:37","modified_gmt":"2025-11-20T09:30:37","slug":"acth-stimulation-test","status":"publish","type":"page","link":"https:\/\/laboklin.com\/ch-it\/products\/haematology-clin-chemistry-endocrinology\/function-tests-calculation-formulas\/acth-stimulation-test\/","title":{"rendered":"ACTH Stimulation Test"},"content":{"rendered":"

ACTH Stimulation Test<\/h3>

LABOKLIN Service ID: 141<\/span><\/p>


<\/p>
Diagnosis<\/strong><\/td>

  • Initial diagnosis of Addison\u2019s disease <\/li>
  • Iatrogenic Cushing\u2019s disease <\/li>
  • Cushing\u2019s disease <\/li>
  • Horse: to clarify Addison\u2019s disease \/iatrogenic Cushing\u2019s disease<\/li><\/ul><\/p><\/td><\/tr>
Material<\/strong><\/td>

serum 2 x 0.5 ml<\/p><\/td><\/tr>

Execution<\/strong><\/td>

  • First blood collection = baseline value<\/li>
  • Dog:<\/strong> injection of 5 \u03bcg ACTH\/kg as Cosacthen\u00ae i.v.\/i.m.<\/li>
  • Cat: <\/strong>injection of 5 \u03bcg\/kg Cosacthen\u00ae i.v.\/i.m. (Addison\u2019s disease only)<\/li>
  • Guinea pig:<\/strong> baseline value cortisol, 2 IU ACTH\/kg, 2. sample collection after 4 hours
  • Horse: <\/strong>injection of 100 IU ACTH i.v. (only hypoadrenocorticism)<\/li>
  • Second blood collection 1 hour post ACTH injection = stimulation value in dogs and cats <\/li>
  • Second blood collection 2 hours post ACTH administration = stimulation value in horses<\/li> <\/ul><\/p><\/td><\/tr>
Parameter<\/strong><\/td>

Cortisol<\/p><\/td><\/tr>

Method<\/strong><\/td>

CLIA<\/p><\/td><\/tr>

Species list<\/strong><\/td>

dog, cat, horse, guinea pig, others on request<\/p><\/td><\/tr>

Duration<\/strong><\/td>

1 day<\/p><\/td><\/tr>

Assessment<\/strong><\/td>

  • Addison\u2019s disease\/iatrogenic Cushing\u2019s disease: cortisol concentration post stimulation < 10 ng\/ml (20 ng\/ml in 8% of the cases, in central Addison\u2019s disease a moderate stimulation > 20 ng\/ml can be expected in dogs).<\/li>
  • In Cushing\u2019s disease (hyperadrenocorticism), the cortisol concentration post stimulation exceeds 150 ng\/ml or is more than 3 times the baseline value, as long as it is in the middle of the normal range. Chronic stress and other underlying diseases (e.g. diabetes mellitus) can also lead to an abnormal ACTH response. According to the literature, a stimulation value > 220 ng\/ml is, to a very high percentage, associated with Cushing\u2019s disease. <\/li>
  • It should be noted that approx. 15 % of the dogs with pituitary and approx. 40% of the dogs with adrenal hyperadrenocorticism show a normal, i.e. not significantly elevated increase.<\/li>
  • For the interpretation of therapy monitoring in Cushing's disease see Vetoryl Therapy Control (see category Endocrinology)<\/li>
  • Horse:<\/strong> In healthy animals, the cortisol level increases by approximately 80 %; horses with hypoadrenocorticism show very low baseline values which do not or only slightly increase after stimulation. )<\/li>
  • Guinea pig: <\/strong> for therapy monitoring of Cushing\u2019s disease; at the time of diagnosis, it is not possible to differentiate between pituitary and adrenal Cushing\u2019s disease; there are no reference values available.<\/li><\/ul><\/p><\/td><\/tr>
Remark<\/strong><\/td>

An ACTH Stimulation Test can also be performed in guinea pigs from saliva (test number 1329 Salivary Cortisol Stimulation Test). Sample tubes are provided; saliva samples are analysed using ELISA detection method.<\/p><\/td><\/tr><\/table>\n","protected":false},"excerpt":{"rendered":"

ACTH Stimulation TestLABOKLIN Service ID: 141DiagnosisInitial diagnosis of Addison\u2019s disease Iatrogenic Cushing\u2019s disease Cushing\u2019s disease Horse: to clarify Addison\u2019s disease \/iatrogenic Cushing\u2019s diseaseMaterialserum 2 x 0.5 mlExecution First blood collection = baseline value Dog: injection of 5 \u03bcg ACTH\/kg as Cosacthen\u00ae i.v.\/i.m. Cat: injection of 5 \u03bcg\/kg Cosacthen\u00ae i.v.\/i.m. (Addison\u2019s disease only) Guinea pig: baseline…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1346871,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1418200","page","type-page","status-publish","hentry","description-off"],"_links":{"self":[{"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/pages\/1418200","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/comments?post=1418200"}],"version-history":[{"count":1,"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/pages\/1418200\/revisions"}],"predecessor-version":[{"id":1524239,"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/pages\/1418200\/revisions\/1524239"}],"up":[{"embeddable":true,"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/pages\/1346871"}],"wp:attachment":[{"href":"https:\/\/laboklin.com\/ch-it\/wp-json\/wp\/v2\/media?parent=1418200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}